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SARS-CoV-2 Trimeric Spike Californian B.1.427

 

Figure 1.  Simply Blue stained SDS-PAGE analysis of SARS-CoV-2 Trimeric Spike Californian B.1.427. 4-12% gradient gel is used for analysis. Lane 1. 0.8 µg SARS-CoV-2 Trimeric Spike Californian B.1.427 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 0.8 µg SARS-CoV-2 Trimeric Spike Californian B.1.427 (+DTT).

       

Figure 2. HPLC analytical SEC for final product.

       

Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.

       

Figure 4. Octet Red96e analysis of SARS-CoV-2 Trimeric Spike Californian B.1.427 binding to human ACE2 receptor.

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Quantity:

€ 1950

Catalogue #

P-354-100

Name

SARS-CoV-2 Trimeric Spike Californian B.1.427

Description

Protein contains amino acids 14-1211 and following mutations: W152C, L452R, D614G. Plus two extra amino acids (AS) in N-terminus, trimerization domain and His-6 tag at C-terminus, GS linker between protein and trimerization domain and GSG linker between trimerization domain and His-tag. Furin cleavage (RRAR) site between Spike S1 and S2 is mutated (GSAS).

Uniprot ID

P0DTC2

Source

Human

MW

410.645 kDa

Host

CHO-based cell line (expressed by QMCF Technology)

Purification

Purified by Ni-affinity chromatography and gel-filtration from serum-free CHO growth media, sterile filtrated

Purity

>95%

Concentration

1 mg/ml

Buffer

PBS pH 7.4

Endotoxine

NA

QC

SDS-PAGE, analytical SEC, Octet binding to ACE2 receptor

Shipping

Shipped on dry ice.

Storage

Store at -20 °C to -70 °C long term. Avoid multiple freeze-thaw cycles.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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