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Figure 1. Simply Blue stained SDS-PAGE analysis of SARS-CoV-2 Trimeric Spike Brazil P1 . 4-12% gradient gel is used for analysis. Lane 1. 1.3 µg SARS-CoV-2 Trimeric Spike Brazil P1 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 1.3 µg SARS-CoV-2 Trimeric Spike Brazil P1 (+DTT).
Figure 2. HPLC analytical SEC for final product.
Figure3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet Red96e analysis of SARS-CoV-2 Trimeric Spike Brazil P1 binding to human ACE2 receptor.
Catalogue #
P-318-100
Name
SARS-CoV-2 Trimeric Spike Brazil P1 (gamma)
Description
Protein contains amino acids 14-1211 and following mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F. Plus two extra amino acids (AS) in N-terminus, trimerization domain and His-6 tag at C-terminus, GS linker between protein and trimerization domain and GSG linker between trimerization domain and His-tag. Furin cleavage (RRAR) site between Spike S1 and S2 is mutated (GSAS). Also, two stabilizing prolines (PP) have been added to the S2 domain.
Uniprot ID
P0DTC2
MW
411 kDa
Host
CHO-based cell line (expressed by QMCF Technology)
Purification
Purified by Ni-affinity chromatography and gel-filtration from serum-free CHO growth media, sterile filtrated
Purity
>95%
Concentration
1 mg/ml
Buffer
PBS pH 7.4
Endotoxine
NA
Bioproperties
Measured by its binding ability to ACE2 protein by OCTET RED96 system.
QC
SDS-PAGE, analytical SEC, Octet binding to ACE2 receptor
Shipping
Shipped on dry ice.
Storage
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
© 2023 Icosagen
