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SARS-CoV-2 Trimeric Spike Brazil P1 (gamma)

 

Figure 1.  Simply Blue stained SDS-PAGE analysis of SARS-CoV-2 Trimeric Spike Brazil P1 . 4-12% gradient gel is used for analysis. Lane 1. 1.3 µg SARS-CoV-2 Trimeric Spike Brazil P1 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 1.3 µg SARS-CoV-2 Trimeric Spike Brazil P1 (+DTT).

       

Figure 2. HPLC analytical SEC for final product.

       

Figure3. HPLC analytical SEC after 3 freeze-thaw cycles.

         

Figure 4. Octet Red96e analysis of SARS-CoV-2 Trimeric Spike Brazil P1 binding to human ACE2 receptor.

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Quantity:

€ 1950

Catalogue #

P-318-100

Name

SARS-CoV-2 Trimeric Spike Brazil P1 (gamma)

Description

Protein contains amino acids 14-1211 and following mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F. Plus two extra amino acids (AS) in N-terminus, trimerization domain and His-6 tag at C-terminus, GS linker between protein and trimerization domain and GSG linker between trimerization domain and His-tag. Furin cleavage (RRAR) site between Spike S1 and S2 is mutated (GSAS). Also, two stabilizing prolines (PP) have been added to the S2 domain.

Uniprot ID

P0DTC2

MW

411 kDa

Host

CHO-based cell line (expressed by QMCF Technology)

Purification

Purified by Ni-affinity chromatography and gel-filtration from serum-free CHO growth media, sterile filtrated

Purity

>95%

Concentration

1 mg/ml

Buffer

PBS pH 7.4

Endotoxine

NA

Bioproperties

Measured by its binding ability to ACE2 protein by OCTET RED96 system.

QC

SDS-PAGE, analytical SEC, Octet binding to ACE2 receptor

Shipping

Shipped on dry ice.

Storage

Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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