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Figure 1. Simply Blue Safe stained SDS-PAGE analysis of SARS-CoV-2 Spike S1 Omicron BA.5. 4-12% gradient gel is used for analysis. Lane 1. 0.8 µg SARS-CoV-2 Spike S1 Omicron BA.5 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 0.8 µg SARS-CoV-2 Spike S1 Omicron BA.5 (+DTT) 
Figure 2. HPLC analytical SEC for final product. 
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles. 
Figure 4. Octet RED96e analysis of SARS-CoV-2 S1 Omicron protein binding to the ACE2 receptor.Catalogue #
P-380-100
Name
SARS-CoV-2 Spike S1 Omicron BA.5
Description
Protein contains amino acids 14-681, mutations T19I, L24S, del25/27, del69-70, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, two extra amino acids (AS) in N-terminus, His-6 tag at C-terminus and GSG linker between protein and tag.
Uniprot ID
P0DTC2
MW
75.381 kDa
Host
CHO-based cell line (expressed by QMCF Technology)
Dissociation constant (KD)
2.03E-08 (measured against ACE2 receptor)
Purification
Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.
Purity
>95%
Concentration
1 mg/ml
Buffer
PBS pH 7.4
Endotoxine
NA
QC
Simply Blue Safe stained SDS-PAGE, analytical SEC, Octet Binding to ACE2 receptor
Shipping
Shipped on dry ice
Storage
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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