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Figure 1. Coomassie-stained SDS-PAGE analysis of SARS-CoV-2 Spike S1 Indian B.1.617.1. 4-12% gradient gel is used for analysis. Lane 1. 0.8 µg SARS-CoV-2 Spike S1 Indian B.1.617.1 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 0.8 µg SARS-CoV-2 Spike S1 Indian B.1.617.1 (+DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Binding analysis of the SARS-CoV-2 Spike S1 Indian B.1.617.1 protein to hACE2 receptor. hACE2 -Fc protein was bound to Protein G sensor. 200 nanomols of SARS-CoV-2 Spike S1 protein was bound to the ACE2 receptor.
Catalogue #
P-348-100
Name
SARS-CoV-2 Spike S1 Indian B.1.617.1 (kappa)
Description
Protein contains amino acids 14-681, mutations G142D, E154K, L452R, E484Q, D614G, P681R, two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus and GSG linker between protein and tag.
MW
75.99 kDa
Host
CHO-based cell line (expressed by QMCF Technology)
Purification
Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.
Purity
>95%
Concentration
1 mg/ml
Buffer
PBS pH 7.4
Endotoxine
NA
Bioproperties
Measured by its binding ability to ACE2 protein by OCTET RED96 system.
QC
SDS-PAGE
NanoDrop A280, analytical SEC, Octet binding to ACE2 receptor
Shipping
Shipped on dry ice
Storage
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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