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Figure 1. IF testing of anti-MANF monoclonal antibody (341-100). Immunofluorescence detection of human MANF expressed in human U2OS cells by chimeric monoclonal antibody to hMANF (341-100). Anti-hMANF antibody dilution in IF experiment was 1 µg/ml. Upper panel: MANF-expressing U2OS cells. Lower panel: Negative control (non-transfected cells)
A
B
Figure 2: Immunohistochemical analysis of MANF monoclonal antibody (341-100). Analysis was performed using mouse pancreas cryosections. Antibody concentration of 5 µg/ml was used. A. WT mouse pancreas. B. MANF KO mouse pancreas.
Catalogue #
341-100
Name
Recombinant mAb to human MANF (clone 2B8, chicken-mouse IgG2a chimeric antibody)
Target
Human MANF
Target Description
Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant
Alternative Names
ARMET, ARP
Uniprot ID
P55145
Clonality
Mouse monoclonal
Clone
2B8, chicken-mouse IgG2a chimeric antibody
Class
mIgG2a
Reactivity
Human MANF
Application
ELISA, IF, IHC
Protocol
ELISA:
0,02-0,1 µg/ml
WB:
Conformational antibody, not suitable for Western Blot application
IF:
1-20 µg/ml
Purification
MabSelect affinity chromatography
Buffer
PBS pH 7.4, with 0.1% sodium azide
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Background
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
© 2024 Icosagen
