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Recombinant mAb to human MANF (clone 3D4, chicken-mouse IgG2a chimeric antibody)

                 

Figure 1. IF testing of anti-MANF monoclonal antibody (342-100). Immunofluorescence detection of human MANF expressed in human U2OS cells by chimeric monoclonal  antibody to hMANF (342-100). Anti-hMANF antibody dilution in IF experiment was 1 µg/ml. Upper panel: MANF-expressing U2OS cells. Lower panel: Negative control (non-transfected cells)

 

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Figure 2: Immunohistochemical analysis of MANF monoclonal antibody (342-100). Analysis was performed using mouse pancreas cryosections. Antibody concentration of 5 µg/ml was used. A. WT mouse pancreas. B. MANF KO mouse pancreas

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Quantity:

€ 2098

Catalogue #

342-100

Name

Recombinant mAb to human MANF (clone 3D4, chicken-mouse IgG2a chimeric antibody)

Target

Human MANF

Target Description

Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant

Alternative Names

ARMET, ARP

Uniprot ID

P55145

Clonality

Mouse monoclonal

Clone

3D4, chicken-mouse IgG2a chimeric antibody

Class

mIgG2a

Reactivity

Human MANF

Application

ELISA, IF, IHC-C

Protocol

Conformational antibody, not suitable for Western Blot application.

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

0,02-0,1 µg/ml

WB:

Conformational antibody, not suitable for Western Blot application

IF:

1-20 µg/ml

IHC-C:

5 µg/ml

Purification

MabSelect affinity chromatography

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

Background

MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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+1 415 548 6625

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