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Figure 1. Simply Blue Safe stained SDS-PAGE analysis of recombinant anti-human interleukin-6 antibody, clone 4G5. 4-12% gel is used for analysis. Lane 1. Recombinant anti-human interleukin-6 antibody, clone 4G5 (-DTT) Lane 2. Size marker Lane 3. Recombinant anti-human interleukin-6 antibody, clone 4G5 (+DTT) Lane 4. Size marker
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet BLI analysis, antibody was loaded on sensor for capture of IL-6 protein.Catalogue #
R1-642-100
Name
Recombinant anti-human interleukin-6 antibody (clone 4G5)
Target
Recombinant hIL-6 protein
Target Description
Recombinant hIL-6 protein produced by CHO-based cell line (expressed by QMCF Technology).
Uniprot ID
P05231
Clonality
Human monoclonal
Clone
4G5
Class
hIgG1
Reactivity
Reacts with human IL-6.
No cross-reactivity with IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-8, IFNγ and TNFα proteins.
Application
ELISA, CLIA
Kd:
1.44 x 10-08 M
Purification
Protein A affinity chromatography following desalting
Buffer
PBS pH 7.4
QC
Simply Blue Safe stained SDS-PAGE, analytical SEC, Octet binding
Shipping
Shipped with blue ice.
Storage
Store at +4 °C.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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