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Figure 1. Simply Blue Safe stain analysis of recombinant anti-human interleukin-6 antibody, clone 14E2. 4-12% Nupage gel is used for analysis. Lane 1. Recombinant anti-human interleukin-6 antibody, clone 14E2 (-DTT). Lane 2. Size marker. Lane 3. Recombinant anti-human interleukin-6 antibody, clone 14E2 (+DTT).
Figure 2. HPLC analytical SEC for final product. 
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles. 
Figure 4. Octet BLI analysis.Catalogue #
R1-648-100
Name
Recombinant anti-human interleukin-6 antibody (clone 14E2)
Target
Recombinant hIL-6 protein
Target Description
Recombinant hIL-6 protein produced by CHO-based cell line (expressed by QMCF Technology).
Uniprot ID
P05231
Clonality
Human monoclonal
Clone
14E2
Class
hIgG1
Reactivity
Reacts with human IL-6.
No cross-reactivity with IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-8, IFNγ and TNFα proteins.
Dissociation constant (KD)
1.13 x 10-9 M
Application
ELISA
Kd:
1.13 x 10-09 M
Purification
Protein A affinity chromatography following desalting
Buffer
PBS pH 7.4
QC
Antigen recognition in ELISA, Simply Blue Safe stained SDS-PAGE, analytical SEC, Octet BLI analysis
Shipping
Shipped with blue ice.
Storage
Store at +4 °C.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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