Search from website

Rabbit pAb to human Ribonuclease 8

           

Figure 1. Western Blot analysis of polyclonal RNase 8 antibody. Human RNase 8 was expressed by CHOEBNALT85 cell line. 50 ng of purified protein was loaded per line. Line 1. 50 ng non-reduced RNase 8; Line 2. Prestained Protein Ladder, Naxo 8003. Primary antibody concentration 1 µg/ml was used for analysis.

     

 

Figure 2. Immunofluorescence detection of human RNase 8 expressed in U2OS cells by anti-RNase 8 polyclonal antibody. Anti-RNase 8 antibody  concentration in IF experiment was 1 µg/ml. Goat ant-rabbit AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for RNase 8 specific signal and blue for nuclei. A. Human RNase 8 - expressing U2OS cells; B. Negative control (non-transfected U2OS cells).

Select volume:

Quantity:

€ 2098

Catalogue #

430-100

Name

Rabbit pAb to human Ribonuclease 8

Target

Human Ribonuclease 8

Target Description

Recombinant human RNase 8 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line

Alternative Names

RNase 8

Uniprot ID

Q8TDE3

Clonality

Polyclonal

Class

IgG

Reactivity

human RNase 8, cross-reactivity to human RNase 7

Application

ELISA, WB, IF

Protocol

Antibody optimal dilution has to be established practically for each antigen and assay format

ELISA:

1-2 µg/ml

WB:

0,1-0,5 µg/ml

IF:

1-10 µg/ml

Purification

RNase 8 - affinity purified

Buffer

Concentrated ammonium sulphate in PBS pH 7.4

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use

Background

The RNase 8 gene is linked to seven other RNase A superfamily genes on chromosome 14. It is expressed prominently in the placenta, but is not detected in any other tissues examined. The ribonucleolytic activity of recombinant human RNase 8 is among the lowest of members of this superfamily and it exhibits neither antiviral nor antibacterial activities characteristic of some other RNase A ribonucleases (reviewed from Zhang et al. 2002, NAR, 30 (5) 1169-1175).

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

Our locations

United States Office

77 Geary St. 5th Floor
San Francisco, CA 94108
+1 415 548 6625

European Office

Eerika tee 1
Õssu, Kambja vald
Tartu maakond 61713
Estonia
+372 737 7070
error:
Shopping Cart