Search from website

Rabbit polyclonal antibody to human CDNF

Figure 1. Western Blot testing of anti-CDNF polyclonal antibody. Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Recombinant CDNF expressed into the supernatant of CHO cell culture medium.

A B

Figure 2. Immunohistochemistry testing of anti-CDNF rabbit polyclonal antibody. Analysis was performed using formalin-fixed paraffin-embedded human cerebral cortex tissue sections from Alzheimer’s disease patients. Tissue sections were boiled with sodium citrate buffer (pH 6) for antigen retrieval. Incubation with primary antibody at 5 µg/ml was performed overnight at 4°C. DAKO EnVisionTM Detection System, Peroxidase/DAB was used for visualization. Sections were counterstained with toluidine blue and mounted with Eukitt mounting medium. A. CDNF staining by rabbit polyclonal anti-CDNF antibody; B. Negative staining without primary antibody. A B
Figure 3. Immunofluorescence  detection of human CDNF expressed in U2OS cells. CDNF was visualized using anti-CDNF rabbit polyclonal antibody, dilution 1:3000. Goat ant-rabbit AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for CDNF specific signal and blue for nuclei. A. CDNF-expressing U2OS cells; B. Negative control (non-transfected U2OS cells).

Select volume:

Quantity:

€ 2098

Catalogue #

300-100

Name

Rabbit polyclonal antibody to human CDNF

Target

Human CDNF

Target Description

Recombinant human CDNF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Purified from cell culture supernatant.

Alternative Names

ARMETL1

Uniprot ID

Q49AH0

Clonality

Polyclonal

Class

IgG

Reactivity

Human, mouse; others not tested

Application

ELISA, WB, IF, IHC-P

Protocol

Monoclonal antibody working dilution has to be established practically for each particular antigen and assay format

ELISA:

0,1-0,2 µg/ml

WB:

0,1-0,2 µg/ml

IF:

0,3-2 µg/ml

IHC-P:

5-10 µg/ml (on formalin fixed, paraffin-embedded tissues, antigen retrieval)

Purification

CDNF-affinity purification

Buffer

50% saturated ammonium sulphate in PBS pH 7.4

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at +4°C upon receipt. As product is (NH 4)2SO4 precipitate, mix well by pipetting or vortexing prior use

Background

CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com
error:
Shopping Cart