Rabbit polyclonal antibody to human CDNF

Catalogue #

300-100

Name

Rabbit polyclonal antibody to human CDNF

Target

Human CDNF

Target Description

Recombinant human CDNF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Purified from cell culture supernatant.

Alternative Names

ARMETL1

Uniprot ID

Q49AH0

Clonality

Polyclonal

Class

IgG

Reactivity

Human, mouse; others not tested

Application

ELISA, WB, IF, IHC-P

Protocol

ELISA:

0,1-0,2 µg/ml

WB:

0,1-0,2 µg/ml

IF:

0,3-2 µg/ml

IHC-P:

5-10 µg/ml (on formalin fixed, paraffin-embedded tissues, antigen retrieval)

Purification

CDNF-affinity purification

Buffer

Ammonium sulphate, saturated (PBS pH 7.4)

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at +4°C upon receipt. As product is (NH ₄)₂SO₄ precipitate, mix well by pipetting or vortexing prior use

Background

CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta