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Rabbit polyclonal antibody to IgY, HRP conjugate

Figure 1. Western blot analysis for testing of Rabbit anti-chicken polyclonal antibody HRP conjugate as secondary antibody. Chicken anti-MANF polyclonal antibody (Icosagen) was used as primary antibody.
For secondary antibody testing purified human MANF protein was used. Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Purified MANF protein. 100 ng of protein is loaded per lane

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Quantity:

€ 2098

Catalogue #

903-100

Name

Rabbit polyclonal antibody to IgY, HRP conjugate

Target

chicken IgY

Target Description

Purified IgY fraction

Class

IgG

Reactivity

Reacts with chicken IgY antibodies

Application

ELISA, WB

Protocol

Polyclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

0,03-0,05 µg/ml

WB:

0,1-0,2 µg/ml

Purification

IgG fraction purifiedby IgY affinity chromatography

Buffer

PBS pH 7.4, with 50% glycerol

Shipping

This product is shipped in frozen format

Storage

Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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