Mouse mAb to human Ribonuclease 7 (clone 3C2)

Catalogue #

406-100

Name

Mouse mAb to human Ribonuclease 7 (clone 3C2)

Target

Human Ribonuclease 7

Target Description

Recombinant human RNase 7 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line

Alternative Names

RNase 7, SAP-2 (skin-derived antimicrobial protein 2)

Uniprot ID

Q9H1E1

Clonality

Mouse monoclonal

Clone

3C2

Class

mIgG1

Reactivity

Human Ribonuclease7

Application

ELISA, WB, IF, IHC

Protocol

ELISA:

0,05-0,1 µg/ml

WB:

0,5-1 µg/ml

IF:

2-20 µg/ml

IHC:

5-20 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1 % sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20°C to -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result

Background

RNase 7 exhibit potent ribonuclease activity and thus may contribute to the well-known ribonuclease activity of human skin. RNase 7 revealed broad spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nm) against a vancomycin-resistant Enterococcus faecium (Harder and Schröder, 2002)