Search from website

Mouse mAb to human Ribonuclease 7 (clone 3C2)

Figure 1. Western Blot analysis of RNase 7 antibody 3C2. Human RNase 7 was expressed by CHOEBNALT85 cell line. 10 µl of cell culture supernatant was loaded per line. Line 1. Prestained Protein Ladder, Naxo 8003; Line 2. non-reduced conditions; Line 3. Reduced conditions.
Figure 2. Immunohistochemistry analysis of of RNase 7 antibody 3C2. Analysis was performed using paraffin-embedded human skin tissue sample. Anti-RNase 7 antibody 3C2 was used as primary antibody. Biotinylated anti-mouse antibody was used as secondary antibody. Streptavidin-HRP conjugate was used for visualization.

Select volume:

Quantity:

€ 2098

Catalogue #

406-100

Name

Mouse mAb to human Ribonuclease 7 (clone 3C2)

Target

Human Ribonuclease 7

Target Description

Recombinant human RNase 7 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line

Alternative Names

RNase 7, SAP-2 (skin-derived antimicrobial protein 2)

Uniprot ID

Q9H1E1

Clonality

Mouse monoclonal

Clone

3C2

Class

mIgG1

Reactivity

Human Ribonuclease7

Application

ELISA, WB, IF, IHC

Protocol

Monoclonal antibody optimal dilution has to be established practically for each antigen and assay format

ELISA:

0,05-0,1 µg/ml

WB:

0,5-1 µg/ml

IF:

2-20 µg/ml

IHC:

5-20 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1 % sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20°C to -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result

Background

RNase 7 exhibit potent ribonuclease activity and thus may contribute to the well-known ribonuclease activity of human skin. RNase 7 revealed broad spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nm) against a vancomycin-resistant Enterococcus faecium (Harder and Schröder, 2002)

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

Our locations

United States Office

77 Geary St. 5th Floor
San Francisco, CA 94108
+1 415 548 6625

European Office

Eerika tee 1
Õssu, Kambja vald
Tartu maakond 61713
Estonia
+372 737 7070
error:
Shopping Cart