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Mouse mAb to human Ribonuclease 7 (clone 4C4)

Figure 1. Western Blot analysis of RNase 7 antibody 4C4. Human RNase 7 was expressed by CHOEBNALT85 cell line. 10 µl of cell culture supernatant was loaded per lane. Lane 1 non-reduced conditions; Lane 2 Reduced conditions; Lane 3 Prestained Protein Ladder, Naxo 8003

   
 
A.                                 B.
 
Figure 2. Immunofluorescence testing anti-RNase7 monoclonal antibody 4C4. Assay was carried out with U2OS cells expressing RNase 7. Antibody concentration of 0.5 ug/ml was used. Nuclei were counterstained by DAPI. A. U2OS cells expressing RNase 7; B. Negative control (non-transfected U2OS cells)
 
Figure 3. Immunohistochemistry analysis of RNase 7 antibody 4C4. Analysis was performed using paraffin-embedded human skin tissue sample. Anti-RNase 7 antibody 4C4 (dilution 1:500) was used as primary antibody. Biotinylated anti-mouse antibody was used as secondary antibody. Streptavidin-HRP conjugate was used for visualization

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Quantity:

€ 2098

Catalogue #

407-100

Name

Mouse mAb to human Ribonuclease 7 (clone 4C4)

Target

Human Ribonuclease 7

Target Description

Recombinant human RNase 7 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line

Alternative Names

RNase 7, SAP-2 (Skin-derived antimicrobial protein 2)

Uniprot ID

Q9H1E1

Clonality

Mouse monoclonal

Clone

4C4

Class

mIgG1

Reactivity

Human Ribonuclease7

Application

ELISA, WB in non-reduced conditions; IHC,IF

Protocol

Monoclonal antibody optimal dilution has to be established practically for each antigen and assay format

ELISA:

0,05-0,1 µg/ml

WB:

0,5-1 µg/ml

IF:

0,2-20 µg/ml

IHC:

2-20 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result

Background

RNase 7 exhibit potent ribonuclease activity and thus may contribute to the well-known ribonuclease activity of human skin. RNase 7 revealed broad spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nm) against a vancomycin-resistant Enterococcus faecium (Harder and Schröder, 2002)

References

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This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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