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Figure 1.Western Blot analysis of RNase 7 antibody 4F9. Human RNase 7 was expressed by CHOEBNALT85 cell line. 10 µl of cell culture supernatant was loaded per line. Line 1. Prestained Protein Ladder, Naxo 8003; Line 2. non-reduced conditions; Line 3. Reduced conditions
Figure 2.Immunohistochemistry analysis of of RNase 7 antibody 4F9. Analysis was performed using paraffin-embedded human skin tissue sample. Anti-RNase 7 antibody 4F9 1:500 was used as primary antibody. Biotinylated anti-mouse antibody was used as secondary antibody. Streptavidin-HRP conjugate was used for visualization
Catalogue #
408-100
Name
Mouse mAb to human Ribonuclease 7 (clone 4F9)
Target
Human Ribonuclease 7
Target Description
Recombinant human RNase 7 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line
Alternative Names
RNase 7, SAP-2 (Skin-derived antimicrobialprotein 2)
Uniprot ID
Q9H1E1
Clonality
Mouse monoclonal
Clone
4F9
Class
mIgG1
Reactivity
Human Ribonuclease7
Application
ELISA,WB in non-reduced conditions, IHC, IF
Protocol
ELISA:
0,02-0,05 µg/ml
WB:
0,5-1 µg/ml
IF:
1-10 µg/ml
IHC:
5-20 µg/ml
Purification
Protein G purification
Buffer
PBS pH 7.4, with 0.1 % sodium azide
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at -20 °C to -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result
Background
RNase 7 exhibit potent ribonuclease activity and thus may contribute to the well-known ribonuclease activity of human skin. RNase 7 revealed broad spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nm) against a vancomycin-resistant Enterococcus faecium (Harder and Schröder, 2002)
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com