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Figure 1. Western-Blot detection of human NRTN expressed in CHO cells. Line 1. hNRTN-containing CHOEBNALT85 cell culture supernatant. Line 2. Negative control - CHOEBNALT85 cell culture supernatant. 5 µl of supernatant were loaded per line. Primary antibody dilution 1:5000 was used. AP-conjugated Goat-anti mouse, was used as secondary antibody. Antibody recognizes protein dimeric, and monomeric variants
Catalogue #
321-100
Name
Mouse mAb to hNRTN (clone 4G8)
Target
human Neurturin
Target Description
Recombinant human NRTN protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Protein was purified from the cell culture supernatant
Alternative Names
NRTN, NTN
Uniprot ID
Q99748
Clonality
Mouse monoclonal
Clone
4G8
Class
mIgG1
Reactivity
Human NRTN
Application
ELISA, WB, IF
Protocol
ELISA:
0,05-0,1 µg/ml
WB:
0,2-1 µg/ml
IF:
2-10 µg/ml
Purification
Protein G purification
Buffer
PBS pH 7.4, with 0.1% sodium azide
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result
Background
Neurturin is a member of the GDNF family of ligands. GDNF family proteins are distant members of the TGF-beta superfamily
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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