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Mouse mAb to human MANF (clone 1G12)

Mouse mAb to human MANF (clone 1G12)                

Figure 1. Western Blot testing of anti-MANF monoclonal antibody. Line 2. Page Ruler prestained protein ladder, Fermentas; Line 1. Purified recombinant MANF (500 ng per line) expressed using CHOEBNALT85  cell line

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Quantity:

€ 2307

Catalogue #

311-100

Name

Mouse mAb to human MANF (clone 1G12)

Target

Human MANF

Target Description

Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant

Alternative Names

ARMET, ARP

Uniprot ID

P55145

Clonality

Mouse monoclonal

Clone

1G12

Class

mIgG1

Reactivity

Human MANF

Application

ELISA, WB, IF, IHC-C

Protocol

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

0,1-0,2 µg/ml

WB:

0,5-2 µg/ml

IF:

1-10 µg/ml

IHC-C:

5 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form inambient conditions

Storage

Store at -20°C to -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

Background

MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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