Mouse mAb to human laminin a5 (clone 4B12)

Catalogue #

A2-610-100

Name

Mouse mAb to human laminin a5 (clone 4B12)

Target

Human laminin a5

Target Description

Laminin purified from human placenta (Chemicon)

Clonality

Mouse monoclonal

Clone

4B12

Class

mIgG1

Reactivity

Human laminin α5

Application

ELISA, IP, IF

Protocol

ELISA:

0,05-0,1 µg/ml

WB:

This antibody does not work in WB

IP:

0,5 mg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

Background

Laminins are a family of large heterotrimeric proteins that promote cell adhesion and migration via integrins and other cell-surface receptors. They are major components of basement membranes of blood vessels and other tissue compartments, and are synthesized by numerous cell types. Through combination, laminin chains constitute 15 laminin isoforms. Laminin isoforms, particularly their α chain, are expressed in a cell- and tissue-specific manner, and are differentially recognized by integrins

References

Wondimu Z, Geberhiwot T, Ingerpuu S, Juronen E, Xie X, Lindbom L, Doi M, Kortesmaa J, Thyboll J, Tryggvason K, Fadeel B, Patarroyo M. 2004.
An endothelial laminin isoform, laminin-8 (α4β1γ1), is secreted by blood neutrophils, promotes neutrophil migration and extravasation, and protects neutrophils from apoptosis. Blood, 104(6):1859-66