Mouse mAb to human laminin a4 chain (clone 6C3)

Catalogue #

A2-604-100

Name

Mouse mAb to human laminin a4 chain (clone 6C3)

Target

Human laminin a4

Target Description

Immunoaffinity-purified Laminin 411 (laminin-8 (α4β1γ1) from platelets)

Uniprot ID

Q16363

Clonality

Mouse monoclonal

Clone

6C3

Class

mIgG1

Reactivity

Human laminin α4

Application

ELISA, WB, IP

Protocol

ELISA:

0,5-1 µg/ml

WB:

0,5-1 µg/ml reduced conditions

IP:

0,5 mg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

Background

Laminins are a family of large heterotrimeric proteins that promote cell adhesion and migration via integrins and other cell-surface receptors. They are major components of basement membranes of blood vessels and other tissue compartments, and are synthesized by numerous cell types. Through combination, laminin chains constitute 15 laminin isoforms. Laminin isoforms, particularly their α chain, are expressed in a cell- and tissue-specific manner, and are differentially recognized by integrins

References

Wondimu Z, Geberhiwot T, Ingerpuu S, Juronen E, Xie X, Lindbom L, Doi M, Kortesmaa J, Thyboll J, Tryggvason K, Fadeel B, Patarroyo M. 2004.
An endothelial laminin isoform, laminin-8 (α4β1γ1), is secreted by blood neutrophils, promotes neutrophil migration and extravasation, and protects neutrophils from apoptosis. Blood, 104(6):1859-66.

Kawataki T, Yamane T, Naganuma H, Rousselle P, Andurén I, Tryggvason K, Patarroyo M. 2007.
Laminin isoforms and their integrin receptors in glioma cell migration and invasiveness: Evidence for a role of alpha5-laminin(s) and alpha3beta1 integrin. Exp Cell Res