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Mouse mAb to human CDNF (clone 6G5)

Figure 1. Western Blot testing of anti-CDNF monoclonal antibody (6G5). Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas). Line 2. Recombinant CDNF expressed into the supernatant of CHO cell culture medium.

A B

Figure 2. Immunohistochemistry testing of anti-CDNF monoclonal antibody 6G5. Analysis was performed using formalin-fixed paraffin-embedded human cerebral cortex tissue sections from Alzheimer’s disease patients. Tissue sections were boiled with sodium citrate buffer (pH 6) for antigen retrieval. Incubation with primary antibody at 5 µg/ml was performed overnight at 4°C. DAKO EnVisionTM Detection System, Peroxidase/DAB was used for visualization. Sections were counterstained with toluidine blue and mounted with Eukitt mounting medium. A. CDNF staining by monoclonal antibody 6G5; B. Negative staining without primary antibody.

A B

Figure 3. Immunofluorescence  detection of human CDNF expressed in U2OS cells. CDNF was visualized using anti-CDNF antibody clone 6G5 at 1 µg/ml. Goat ant-mouse AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for CDNF specific signal and blue for nuclei. A. CDNF-expressing U2OS cells; B. Negative control (non-transfected U2OS cells).

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Quantity:

€ 2098

Catalogue #

302-100

Name

Mouse mAb to human CDNF (clone 6G5)

Target

Human CDNF

Target Description

Recombinant human CDNF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Purified from cell culture supernatant

Alternative Names

ARMETL1

Uniprot ID

Q49AH0

Clonality

Mouse monoclonal

Clone

6G5

Class

mIgG1

Reactivity

Human, no reactivity with mouse CDNF
Binds to the C-terminal part of the human CDNF (aa 126-187)

Application

ELISA, WB, IF, IHC

Protocol

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

0,06-0,2 µg/ml

WB:

0,25-1 µg/ml

IF:

0,3-10 µg/ml

IHC:

5-10 µg/ml (on formalin fixed, paraffin-embedded tissues)

Purification

Protein G purified

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Background

CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com
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