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Mouse mAb to hBDNF (clone 3C11)

Figure 1. Western Blot testing of anti-BDNF monoclonal antibody 3C11 (5 µg/ml). Different amounts (1, 10 and 100 ng) of  recombinant hBDNF (E. coli produced) was loaded per lane. Photo courtesy of Indrek Koppel and Tõnis Timmusk, Tallinn Technical University, Institute of Gene Technology.
Figure 2. Western Blot testing of anti-BDNF monoclonal antibody 3C11 (5 µg/ml). HRP-conjugated goat anti-mouse antibody was used as secondary antibody. 10 µl of CHO supernatant containing BDNF was loaded per lane.
             

Figure 3. Western Blot testing of anti-BDNF monoclonal antibody 3C11. Antibody concentrations of 1 µg/ml and 0.2 µg/ml was used. 20 µg of cell lysate was loaded per lane. Lanes 1 and 2 – rat astrocytes’ culture preparation. Lanes 3 and 4 – rat hippocampal neuron culture lysate. Photo courtesy of Indrek Koppel and Tõnis Timmusk, Tallinn Technical University, Institute of Gene Technology.

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Quantity:

€ 2098

Catalogue #

327-100

Name

Mouse mAb to hBDNF (clone 3C11)

Target

Human BDNF

Target Description

Recombinant mature human BDNF protein produced in E. coli

Alternative Names

Abrineurin

Uniprot ID

P23560

Clonality

Mouse monoclonal

Clone

3C11

Class

mIgG1

Reactivity

human, mouse, rat, guinea pig

Application

ELISA, WB, IF

Protocol

Monoclonal antibody working amount has to be established practically for each particular antigen and assay format.

ELISA:

0,02-1 µg/ml

WB:

0,1-1 µg/ml under reducing conditions

IF:

2-20 µg/ml

Purification

Protein G purified

Buffer

PBS pH 7.4, with 0.1% sodium azide.

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20… -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.

Background

Brain-derived neurotrophic factor (BDNF) plays an important role in activity-dependent synaptic plasticity such as long-term potentiation. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses

References

Zunino, G. Messina, A. Sgadò, P. Baj, G. Casarosa, S. Bozzi, Y. Brain - derived neurotrophic factor signaling is altered in the forebrain of Engrailed-2 knockout mice.
Neuroscience. 2016 Mar 14

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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