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Mouse mAb to hBDNF (clone 3B2)

Figure 1. Western Blot analysis of anti-BDNF monoclonal antibody 3B2. Lane 1: 10 µl of supernatant was loaded into the gel under reducing conditions. Antibody concentration 5 µg/ml. HRP-conjugated Goat anti-Mouse IgG was used as secondary antibody. Lane 2: Protein size marker Figure 2. Immunohistochemistry testing of anti-BDNF monoclonal antibody 3B2. Analysis was performed using FFPE human cerebral cortex tissue sections from Alzheimer's disease patients. Tissue sections were boiled with sodium citrate buffer (pH 6) for antigen retrieval. Incubation with primary antibody at 5 µg/ml was performed overnight at 4°C. DAKO EnVisionTM Detection System, Peroxidase/DAB was used for visualization. Sections were counterstained with toluidine blue and mounted with Eukitt mounting medium. A. BDNF staining by monoclonal antibody 3B2; B. Negative staining without primary antibody Figure 2. Immunofluorescence detection of hBDNF expression in U2OS cells by anti-BDNF monoclonal antibody 3B2. Antibody concentration 0.33 µg/ml. Goat anti-mouse AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for BDNF specific signal and blue for nuclei. A. proBDNF-expressing U2OS cells; B. Negative control (non-transfected U2OS cells).

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Quantity:

€ 2098

Catalogue #

329-100

Name

Mouse mAb to hBDNF (clone 3B2)

Target

Human BDNF

Target Description

Recombinant human BDNF protein purified from E. coli

Alternative Names

Abrineurin

Uniprot ID

P23560

Clonality

Mouse monoclonal

Clone

3B2

Class

mIgG2b

Reactivity

Human, mouse, rat, guinea pig

Application

ELISA, WB, IF

ELISA:

0,2-1 µg/ml

WB:

0,2-2 µg/ml (under reducing conditions)

IF:

0,33-20 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4 with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20… -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

Background

Brain-derived neurotrophic factor (BDNF) plays an important role in activity-dependent synaptic plasticity such as long-term potentiation. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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