Mouse mAb to hBDNF (clone 3B2)

Figure 1. Western Blot analysis of anti-BDNF monoclonal antibody 3B2. Lane 1: 10 µl of supernatant was loaded into the gel under reducing conditions. Antibody concentration 5 µg/ml. HRP-conjugated Goat anti-Mouse IgG was used as secondary antibody. Lane 2: Protein size marker Figure 2. Immunohistochemistry testing of anti-BDNF monoclonal antibody 3B2. Analysis was performed using FFPE human cerebral cortex tissue sections from Alzheimer's disease patients. Tissue sections were boiled with sodium citrate buffer (pH 6) for antigen retrieval. Incubation with primary antibody at 5 µg/ml was performed overnight at 4°C. DAKO EnVisionTM Detection System, Peroxidase/DAB was used for visualization. Sections were counterstained with toluidine blue and mounted with Eukitt mounting medium. A. BDNF staining by monoclonal antibody 3B2; B. Negative staining without primary antibody Figure 2. Immunofluorescence detection of hBDNF expression in U2OS cells by anti-BDNF monoclonal antibody 3B2. Antibody concentration 0.33 µg/ml. Goat anti-mouse AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for BDNF specific signal and blue for nuclei. A. proBDNF-expressing U2OS cells; B. Negative control (non-transfected U2OS cells).