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Mouse mAb to hBDNF (clone 4C8)

Figure 1. Western Blot analysis of anti-BDNF monoclonal antibody 4C8. Lanes 1 and 3: 50 ng of purified hBDNF protein was loaded into the gel under non-reducing and reducing conditions, respectively. Antibody concentration 1 μg/ml. Lane 2: Protein size marker
   
A.                                 B.
         

Figure 2. Immunofluorescence detection of hBDNF expression in U2OS cells by anti-BDNF monoclonal antibody 4C8. Antibody concentration 20 μg/ml. Goat anti-mouse AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for BDNF specific signal and blue for nuclei. A. proBDNF-expressing U2OS cells; B. Negative control (non-transfected U2OS cells).

               

Figure 3. Western Blot testing of anti-BDNF monoclonal antibody 4C8. Antibody concentrations of 1 µg/ml was used. 2 Lanes 1 recombiant BDNF  1 µg , Lines 2 and 3 – neuron lysates. Photo courtesy of Indrek Koppel and Tõnis Timmusk, Tallinn Technical University, Institute of Gene Technology.

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Quantity:

€ 2098

Catalogue #

328-100

Name

Mouse mAb to hBDNF (clone 4C8)

Target

Human BDNF

Target Description

Recombinant human BDNF protein purified from E. coli

Alternative Names

Abrineurin

Uniprot ID

P23560

Clonality

Mouse monoclonal

Clone

4C8

Class

mIgG1

Reactivity

Human, mouse, rat. guinea pig

Application

ELISA, WB, IF

ELISA:

0,05-0,1 µg/ml

WB:

0,2-1 µg/ml

IF:

20 µg/ml (weak signal)

Purification

Protein G purification

Buffer

PBS with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20… -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.

Background

Brain-derived neurotrophic factor (BDNF) plays an important role in activity-dependent synaptic plasticity such as long-term potentiation. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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