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Mouse mAb to human Artemin (clone 2G7)

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Figure 1. Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin.  Human U2OS cells were transfected with GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin. Artemin bound to the GFRa3-Ret51 receptor complex was detected by anti-Artemin antibody clone 2G7. Goat anti-mouse Dylight 488 conjugated antibody (dilution 1:1000) was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for Artemin and blue for nuclei. A. Artemin bound to the GFRa3-Ret51 receptor complex; B. Negative control - GFRa3-Ret51 receptor expressing U2OS cells

     
 
 

Figure 2. Immunohistochemistry testing of anti-ARTN monoclonal antibody 2G7. Analysis was performed using FFPE human cerebral cortex tissue sections from Alzheimer's disease patients. Tissue sections were boiled with sodium citrate buffer (pH 6) for antigen retrieval. Incubation with primary antibody at 5 µg/ml was performed overnight at 4°C. DAB was used for visualization. Sections were counterstained with toluidine blue.

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Quantity:

€ 2098

Catalogue #

326-100

Name

Mouse mAb to human Artemin (clone 2G7)

Target

Human Artemin

Target Description

Recombinant human Artemin protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant

Alternative Names

Enovin, Neublastin

Uniprot ID

Q5T4W7

Clonality

Mouse monoclonal

Clone

2G7

Class

mIgG1

Reactivity

Human Artemin

Application

ELISA, IF, IHC

Protocol

Conformational antibody, not suitable for Western Blot application.

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

0,2-1 µg/ml

IF:

1-20 µg/ml

IHC:

5 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

Background

Artemin supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex (UniProt)

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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+1 415 548 6625

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