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Figure 1. Western Blot testing of purified recombinant Artemin by anti-Artemin antibody 2D12. Goat anti-mouse IgG HRP conjugate was used as secondary antibody. 15 ml of CHOEBNALT85 Artemin expressing supernatant was loaded per line. Line 1. Reduced conditions; Line 2. Non-reduced conditions; Line 3. Protein marker (8003, Naxo).
Figure 2. Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin. Human U2OS cells were transfected with GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin. Artemin bound to the GFRa3-Ret51 receptor complex was detected by anti-Artemin antibody clone 2D12. Goat anti-mouse Dylight 488 conjugated antibody (dilution 1:1000) was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for Artemin and blue for nuclei. A. Artemin bound to the GFRa3-Ret51 receptor complex; B. Negative control - GFRa3-Ret51 receptor expressing U2OS cells.
Catalogue #
324-100
Name
Mouse mAb hARTN (clone 2D12)
Target
Human Artemin
Target Description
Recombinant human Artemin protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant
Alternative Names
Enovin, Neublastin
Uniprot ID
Q5T4W7
Clonality
Mouse monoclonal
Clone
2D12
Class
mIgG1
Reactivity
Human Artemin
Application
ELISA, WB, IF
Protocol
ELISA:
0,2-2 µg/ml
WB:
0,5-2 µg/ml (detects only dimeric protein in non-reducing conditions)
IF:
0,3-20 µg/ml
Purification
Protein G purification
Buffer
PBS pH 7.4, with 0.1% sodium azide
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Background
Artemin supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex (UniProt)
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
© 2024 Icosagen
