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Mouse mAb to HPV18 E2 (clone 2E7)

             

Figure 1. Western-Blot analysis of HPV-18 E2 protein. WB was was carried out with 2E7 Mab using the lysates of U2OS and Hela cells. Lanes 1 and 4: respective cells transfected with plasmid expressing HPV-18  E2 protein. Lanes 2 and 3: non-transfected U2OS and Hela cells, respectively. Lane 5: marker.

             

Figure 2.  IF analysis of HPV-18 E2 protein in U2OS cells. IF was carried out with Hela cells transfected with plasmid expressing HPV-18 E2 protein. All the nuclei were stained by DAPI.  Green color represents nucleus-localized HPV-18 E2 detected by Mab 2E7

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Quantity:

€ 2098

Catalogue #

A2-401-100

Name

Mouse mAb to HPV18 E2 (clone 2E7)

Target

HPV-18 E2

Target Description

Recombinant full-length HPV-18 E2-protein purified from E. coli

Clonality

Mouse monoclonal

Clone

2E7

Class

IgG

Reactivity

Reacts with HPV-18 E2-protein. Mapped to amino acids 1-83

Application

ELISA, WB, IF

Protocol

This antibody does not work in IP.

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

0,1 µg/ml

WB:

1 µg/ml

IP:

This antibody does not work in IP

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

Background

Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E7 proteins have cellular transforming and trans-activating activities

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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