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Figure 1. Western-Blot analysis of HPV-18 E2 protein. WB was was carried out with 2E7 Mab using the lysates of U2OS and Hela cells. Lanes 1 and 4: respective cells transfected with plasmid expressing HPV-18 E2 protein. Lanes 2 and 3: non-transfected U2OS and Hela cells, respectively. Lane 5: marker.
Figure 2. IF analysis of HPV-18 E2 protein in U2OS cells. IF was carried out with Hela cells transfected with plasmid expressing HPV-18 E2 protein. All the nuclei were stained by DAPI. Green color represents nucleus-localized HPV-18 E2 detected by Mab 2E7
Catalogue #
A2-401-100
Name
Mouse mAb to HPV18 E2 (clone 2E7)
Target
HPV-18 E2
Target Description
Recombinant full-length HPV-18 E2-protein purified from E. coli
Clonality
Mouse monoclonal
Clone
2E7
Class
IgG
Reactivity
Reacts with HPV-18 E2-protein. Mapped to amino acids 1-83
Application
ELISA, WB, IF
Protocol
ELISA:
0,1 µg/ml
WB:
1 µg/ml
IF:
0.33 - 20 µg/ml
IP:
This antibody does not work in IP
Purification
Protein G purification
Buffer
PBS pH 7.4, with 0.1% sodium azide
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Background
Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E7 proteins have cellular transforming and trans-activating activities
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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