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Figure 1. Western-Blot analysis of HPV-11 E7 protein. WB was carried out with 8G6 Mab using the lysates of U20S and Hela cells. Lines 1 and 4 are according cells transfected with plasmid expressing HPV-11 E7 protein, Lines 2 and 3 non-transfected U2OS and Hela cells. Line 5. Protein size marker
Figure 2. IP analysis of HPV-11 E7 protein. Line 1. shows detection of E7 protein by mAB 8G6 in lysate of U20S cells. Line 2. the lower band is precipitated E7, upper bands are light and heavy chain of Mab. Line 3. precipitation without mAB 8G6 Line 4. Non-transfected U2OS cells. Line 5. Protein size marker
Figure 3. IF analysis of HPV-11 E7 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV-11 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus-localized HPV-11 E7 detected by Mab 8G6. IF analysis of E7 protein show similar result also in HeLa cells
Catalogue #
A2-404-100
Name
Mouse mAb to HPV11 E7 (clone 8G6)
Target
HPV-11 E7
Target Description
Recombinant full-length HPV-11 E7-protein purified from E. coli
Clonality
Mouse monoclonal
Clone
8G6
Class
mIgG2a
Reactivity
Reacts with HPV-11 E7-protein. Mapped to amino acids 1-35
Application
ELISA, WB, IF, IP
Protocol
ELISA:
0,045-0,09 µg/ml
WB:
0,9 µg/ml
IF:
Optimal conditions for IF should be determined for each particular application.
IP:
1,8 µg/ml
Purification
Protein G purification
Buffer
PBS pH 7.4, with 0.1% sodium azide
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Background
Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E7 proteins have cellular transforming and trans-activating activities
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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