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Mouse mAb to HPV11 E2 (clone 10E12)

           

Figure 1. Western-Blot analysis of HPV-11 E2 protein. WB was carried out with 10E12 Mab using the lysates of U20S and Hela cells. Lines 1 and 4 are according cells transfected with plasmid expressing HPV-11  E2 protein, lines 2 and 3 Non-transfected   U2OS and Hela cells. Line 5.  Protein size marker

           

Figure 2. IF analysis of HPV-11 E2 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV-11 E2 protein. All the nuclei were stained by DAPI.  Green color represents nucleus-localized HPV-11 E2 detected by Mab 10E12. IF analysis of E2 protein show similar result also in HeLa cells

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Quantity:

€ 2098

Catalogue #

A2-403-100

Name

Mouse mAb to HPV11 E2 (clone 10E12)

Target

HPV-11 E2

Target Description

Recombinant full-length HPV-11 E2-protein purified from E. coli

Clonality

Mouse monoclonal

Clone

10E12

Class

mIgG1

Reactivity

Reacts with HPV-11 native E2-protein. Mapped to amino acids 202-284

Application

ELISA, WB, IF

Protocol

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

This antibody does not work in IP

ELISA:

0,05-0,1 µg/ml

WB:

1 µg/ml

IP:

This antibody does not work in IP

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

Background

Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com
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