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Mouse mAb to HIV-1 Tat (clone N8)

Figure 1. Western Blot testing of HIV-1 recombinant Tat protein. using HIV-1 Tat antibody (clone N8). Lane 1. Recombinant HIV-1 Tat (B subtype), 100 ng. Antibody concentration 2 µg/ml. Lane 2. Protein size marker.

   
Figure 2. Matrix-ChIP analysis of HIV-1 Tat antibody (clone N8). Human 5A8 J-lat T lymphocytes culture was latently infected with HIV and treated with 10 ug/mL PHA.

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Quantity:

€ 2098

Catalogue #

A2-827-100

Name

Mouse mAb to HIV-1 Tat (clone N8)

Target

HIV-1 Tat

Target Description

Recombinant Tat protein of HIV-1 HAN (purified from E. coli)

Clonality

Mouse monoclonal

Clone

N8

Class

mIgG2b

Reactivity

Reacts in ELISA and WB with HIV-1 Tat of HXB2, BH10,IIIB and HAN strains. Mapped to amino acids 59-67 (PQDSQTHQV – B subtype a.a. consensus)

Application

ELISA, WB, ChIP

Protocol

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

0,05-0,1 µg/ml

WB:

2 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20…-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com
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