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Figure 1. Western Blot testing of HIV-1 recombinant Tat protein. using HIV-1 Tat antibody (clone N8). Lane 1. Recombinant HIV-1 Tat (B subtype), 100 ng. Antibody concentration 2 µg/ml. Lane 2. Protein size marker.
Catalogue #
A2-827-100
Name
Mouse mAb to HIV-1 Tat (clone N8)
Target
HIV-1 Tat
Target Description
Recombinant Tat protein of HIV-1 HAN (purified from E. coli)
Clonality
Mouse monoclonal
Clone
N8
Class
mIgG2b
Reactivity
Reacts in ELISA and WB with HIV-1 Tat of HXB2, BH10,IIIB and HAN strains. Mapped to amino acids 59-67 (PQDSQTHQV – B subtype a.a. consensus)
Application
ELISA, WB, ChIP
Protocol
ELISA:
0,05-0,1 µg/ml
WB:
2 µg/ml
Purification
Protein G purification
Buffer
PBS pH 7.4, with 0.1% sodium azide
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at -20…-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com