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Mouse mAb to HIV-1 Tat (clone N3)

 
Figure 1:  Western Blot testing of HIV-1 recombinant Tat protein. using HIV-1 Tat antibody (clone N3). Lane 1. Recombinant HIV-1 Tat (B subtype), 100 ng. Antibody concentration 2 µg/ml.  Lane 2. Protein size marker.
Figure 2. Matrix-ChIP analysis of HIV-1 Tat antibody (clone N3). Human 5A8 J-lat T lymphocytes culture was latently infected with HIV and treated with 10 ug/mL PHA.

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Quantity:

€ 2098

Catalogue #

A2-825-100

Name

Mouse mAb to HIV-1 Tat (clone N3)

Target

HIV-1 Tat

Target Description

Recombinant Tat protein of HIV-1 HXB2 (purified from E. coli)

Clonality

Mouse monoclonal

Clone

N3

Class

IgM

Reactivity

Reacts with HIV-1 Tat of the strains HXB2, BH10, IIIB and HAN. Mapped to amino acids 47-58 (YGRKKRRQRRRA – B subtype a.a. consensus, Tat basic domain)

Application

ELISA, WB, ChIP

Protocol

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

5 µg/ml

WB:

2 µg/ml

Purification

HiTrap IgM purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

References

Jung HJ, Park Y, Hahm KS, Lee DG. 2006.
Biological activity of Tat (47-58) peptide on human pathogenic fungi. Biochem Biophys Res Commun.  345: 222-8.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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