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Mouse mAb to HIV-1 Rev (clone 10)

 

Figure 1:  Western Blot testing of HIV-1 recombinant Rev protein using HIV-1 Rev antibody, clone 10. Line 1. Recombinant HIV-1 Rev (B subtype) Line 2. Protein size marker.

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Quantity:

€ 2098

Catalogue #

A2-832-100

Name

Mouse mAb to HIV-1 Rev (clone 10)

Target

HIV-1 Rev

Target Description

Recombinant Rev protein of HIV-1 BRV (purified from E. coli)

Clonality

Mouse monoclonal

Clone

10

Class

mIgG1

Reactivity

Reacts with HIV-1 Rev of subtype B. Mapped to amino acids 34-40 (TRGARRN – subtype B a.a. consensus)

Application

ELISA, WB, IF, IP, IHC

Protocol

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

0,25-0,5 µg/ml

WB:

2 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

References

Ranki A., A. Lagerstedt, V. Ovod, E. Aavik, and K. Krohn 1994.
Expression kinetics and subcellular localization of HIV-1 regulatory proteins Nef and Tat in established lymphoid cell lines. Arch. Virol., 139:365-378.

Ranki A., M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn 1995.
Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.

Tähtinen, M., Ranki, A., Valle,S.L., Ovod, V., K. Krohn 1997.
B-cell epitopes in HIV-1 Tat and Rev proteins colocalize with T-cell epitopes and with functional domains. Biomed Pharmacother 51:480-7.

Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren 2001.
Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1):46-61

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com
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