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Figure 1: Western Blot testing of HIV-1 recombinant Rev protein using HIV-1 Rev antibody, clone 10. Line 1. Recombinant HIV-1 Rev (B subtype) Line 2. Protein size marker.
Catalogue #
A2-832-100
Name
Mouse mAb to HIV-1 Rev (clone 10)
Target
HIV-1 Rev
Target Description
Recombinant Rev protein of HIV-1 BRV (purified from E. coli)
Clonality
Mouse monoclonal
Clone
10
Class
mIgG1
Reactivity
Reacts with HIV-1 Rev of subtype B. Mapped to amino acids 34-40 (TRGARRN – subtype B a.a. consensus)
Application
ELISA, WB
Protocol
ELISA:
0,25-0,5 µg/ml
WB:
2 µg/ml
Purification
Protein G purification
Buffer
PBS pH 7.4, with 0.1% sodium azide
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
References
Ranki A., A. Lagerstedt, V. Ovod, E. Aavik, and K. Krohn 1994.
Expression kinetics and subcellular localization of HIV-1 regulatory proteins Nef and Tat in established lymphoid cell lines. Arch. Virol., 139:365-378.
Ranki A., M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn 1995.
Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.
Tähtinen, M., Ranki, A., Valle,S.L., Ovod, V., K. Krohn 1997.
B-cell epitopes in HIV-1 Tat and Rev proteins colocalize with T-cell epitopes and with functional domains. Biomed Pharmacother 51:480-7.
Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren 2001.
Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1):46-61
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com