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Mouse mAb to HIV-1 p24 (clone 5)

Mouse mAb to HIV-1 p24 (clone 5)  

Figure 1. Western-Blot analysis of HIV-1 HAN subtype gag protein using HIV-1 p24 antibody. Line 1. Recombinant HIV-1 HAN-2 subtype gag expressed in composition of virus like particles (VLPs) using 293-based cell line. Line 2. Protein size marker.

Mouse mAb to HIV-1 p24 (clone 5)

Figure 2. IF analysis using HIV-1 p24 antibody, clone 5 in detection of 05-001 epitope tagged ETAR (Endothelin A receptor) in HEK293-based cell line.

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Quantity:

€ 2307

Catalogue #

A2-851-100

Name

Mouse mAb to HIV-1 p24 (clone 5)

Target

HIV-1 p24

Target Description

Recombinant p24 protein of HIV-1 HAN consensus (purified from E. coli)

Clonality

Mouse monoclonal

Clone

5

Class

mIgG2a

Reactivity

Reacts with HIV-1 p24 subtype B, C and A. Mapped to amino acids 48-62 (TPQDLNTMLNTVGGH)

Application

ELISA, WB, IF

Protocol

Antibody can be used efficiently for IF application in U2OS, CHO and 293-based cell lines.

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

0,05-0,1 µg/ml

WB:

1 µg/ml

IF:

1 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Related Products

Partially humanized, chimeric antibody to HIV-1 p25 C-151-100, clone 5. HIV-1 p24 monoclonal antibodies.

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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