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Figure 1: Western Blot testing of HIV-1 recombinant Nef protein. using HIV-1 Nef antibody, clone 2F2. Line 1. Recombinant HIV-1 Nef (B subtype) Line 2. Protein size marker.
Catalogue #
A2-803-100
Name
Mouse mAb to HIV-1 Nef (clone 2F2)
Target
HIV-1 Nef
Target Description
Recombinant Nef protein of HIV-1 BRU (purified from E. coli)
Alternative Names
3’ORF
Clonality
Mouse monoclonal
Clone
2F2
Class
mIgG1
Reactivity
Reacts with HIV-1 Nef subtypes B and C; BRU, and IIIB strains. Mapped to amino acids 150-158 (PDKVEEANK, HIV-1 BRU; PEKVEEANE – B subtype consensus)
Application
ELISA, WB
Protocol
ELISA:
0,05-0,1 µg/ml
WB:
2 µg/ml
Purification
Protein G purification
Buffer
PBS pH 7.4, with 0.1% sodium azide
Related Products
HIV-1 Nef monoclonal antibodies. For more information visit www.icosagen.com/products/?antibodies
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
References
Ovod V., Lagerstedt, A., Ranki, A., Gombert, F.O., Spohn, R., Tahtinen, M., Jung, G., Krohn K.J. 1992.
Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS, 6:25-34.
Saito, Y., L. R. Sharer, L. G. Epstein, J. Michaels, M. Mintz, M. Louder, K. Golding, T. A. Cvetkovich, and B. M. Blumberg. 1994.
Overexpression of Nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-481
Ranki A. , M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn. 1995.
Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.
Collings, A., Pitkanen, J., Strengell, M., Tahtinen, M., Lagerstedt, A., Hakkarainen, K., Ovod, V., Sutter, G., Ustav, M., Ustav, E., Mannik, A., Ranki, A., Peterson, P., Krohn, K. 1999.
Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine. 18(5- 6):460-7.
Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren. 2001.
Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com