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Mouse mAb to HIV-1 Nef (clone 3E6)

               

Figure 1.  Western Blot testing of HIV-1 recombinant Nef protein. Using HIV-1 Nef antibody, clone 3E6. Line 1. Recombinant HIV-1 Nef (B subtype) Line 2. Protein size marker.

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Quantity:

€ 2098

Catalogue #

A2-804-100

Name

Mouse mAb to HIV-1 Nef (clone 3E6)

Target

HIV-1 Nef

Target Description

Recombinant Nef protein of HIV-1 B subtype consensus (purified from E. coli)

Alternative Names

3’ORF

Clonality

Mouse monoclonal

Clone

3E6

Class

mIgG1

Reactivity

Reacts with HIV-1 Nef of subtypes A, B and C; BRU,IIIB, LAV, BH10 strains. Mapped to amino acids 163-173 (SLLHPMSLHGM – B subtypes consensus)

Application

ELISA, WB, ICC, RIP

Protocol

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

0,033-1 µg/ml

WB:

1 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide.

Related Products

HIV-1 Nef monoclonal antibodies. For more information visit www.icosagen.com/products/?antibodies

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

References

Ovod V., Lagerstedt, A., Ranki, A., Gombert, F.O., Spohn, R., Tahtinen, M., Jung, G., Krohn K.J. 1992.
Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS, 6:25-34.

Saito, Y., L. R. Sharer, L. G. Epstein, J. Michaels, M. Mintz, M. Louder, K. Golding, T. A. Cvetkovich, and B. M. Blumberg. 1994.
Overexpression of Nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-481

Ranki A. , M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn. 1995.
Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.

Fiala, A.M., R.H. Rhodes, P. Shapshak, I. Nagano, O. Martinez-Maza, A. Diagne, G. Baldwin, and M. Graves. 1996.
Regulation of HIV-1 infection in astrocytes: Expression of NEF, TNF-alpha and IL-6 is enhanced in coculture of astrocytes with macrophages. J.Neurovirology, 2, No. 3: 158-166. PMID: 8799208

Koedel, U., Kohleisen B, Sporer B, Lahrtz F, Ovod V, Fontana A, Erfle V, Pfister HW. 1999.
HIV type 1 Nef protein is a viral factor for leukocyte recruitment into the central nervous system. J Immunol. 1;163(3):1237-45. PMID: 10415019

Collings, A., Pitkanen, J., Strengell, M., Tahtinen, M., Lagerstedt, A., Hakkarainen, K., Ovod, V., Sutter, G., Ustav, M., Ustav, E., Mannik, A., Ranki, A., Peterson, P., Krohn, K. 1999.
Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine. 18(5- 6):460-7.

Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren. 2001.
Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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