Mouse mAb to HIV-1 Nef (clone 3D12)

Catalogue #

A2-802-100

Name

Mouse mAb to HIV-1 Nef (clone 3D12)

Target

HIV-1 Nef

Target Description

Recombinant Nef protein of HIV-1 HXB2 (purified from E. coli)

Alternative Names

3’ORF

Clonality

Mouse monoclonal

Clone

3D12

Class

mIgG1

Reactivity

Reacts with HIV-1 subtypes B and C, BRU, IIIB, and HAN strains. Mapped to amino acids 35-50 (RDLEKHGAITSSNTAA - HXB2)

Application

ELISA, WB

Protocol

ELISA:

0,1-0,2 µg/ml

WB:

2 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20… -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

References

Ovod V., Lagerstedt, A., Ranki, A., Gombert, F.O., Spohn, R., Tahtinen, M., Jung, G., Krohn K.J. 1992.
Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS, 6:25-34.

Saito, Y., L. R. Sharer, L. G. Epstein, J. Michaels, M. Mintz, M. Louder, K. Golding, T. A. Cvetkovich, and B. M. Blumberg. 1994.
Overexpression of Nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-481

Ranki A. , M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn. 1995.
Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.

Collings, A., Pitkanen, J., Strengell, M., Tahtinen, M., Lagerstedt, A., Hakkarainen, K., Ovod, V., Sutter, G., Ustav, M., Ustav, E., Mannik, A., Ranki, A., Peterson, P., Krohn, K. 1999.
Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine. 18(5- 6):460-7.

Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren. 2001.
Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61.

Maksiutov A.Z. , A.G. Bachinskii, and S.I. Bazhan. 2002
[Searching for Local Similarities Between HIV-1 and Human Proteins. Application to Vaccines]. Mol Biol (Mosk), 36(3):447-459.

Lehmann M.H., Walter S, Ylisastigui L, Striebel F, Ovod V, Geyer M, Gluckman JC, Erfle V. 2006.
Extracellular HIV-1 Nef increases migration of monocytes. Exp Cell Res. 312 (18):3659-68.