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Mouse mAb to HIV-1 Nef (clone 2H12)


Figure 1. Western Blot testing of recombinant HIV-1 B subtype Nef protein. Using HIV-1 Nef antibody (clone 2H12). Lane 1. Recombinant HIV-1 Nef (B subtype) Lane 2. Protein size marker.

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€ 2307

Catalogue #



Mouse mAb to HIV-1 Nef (clone 2H12)


HIV-1 Nef

Target Description

Recombinant Nef protein of HIV-1 BRU (purified from E. coli)

Alternative Names



Mouse monoclonal






Reacts with HIV-1 Nef of B and C subtypes, BRU, IIIB, and HAN strains
Epitope: mapped to amino acids 171-190 (-HGMDDPEREVLEWRFDSRLA-) (HIV-1BRU)




0,027-0,8 µg/ml


0,8 µg/ml


Protein G purification


PBS pH 7.4, with 0.1% sodium azide

Related Products

HIV-1 Nef monoclonal antibodies. For more information visit


This product is shipped in non-frozen liquid form in ambient conditions


Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles


Ovod V., Lagerstedt, A., Ranki, A., Gombert, F.O., Spohn, R., Tahtinen, M., Jung, G., Krohn K.J. 1992.
Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS, 6:25-34.

Saito, Y., L. R. Sharer, L. G. Epstein, J. Michaels, M. Mintz, M. Louder, K. Golding, T. A. Cvetkovich, and B. M. Blumberg. 1994.
Overexpression of Nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-481

Ranki A. , M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn. 1995.
Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.

Koedel, U. , Kohleisen B, Sporer B, Lahrtz F, Ovod V, Fontana A, Erfle V, Pfister HW. 1999.
HIV type 1 Nef protein is a viral factor for leukocyte recruitment into the central nervous system. J Immunol. 163(3):1237-45.

Kohleisen, B. , Hutzler P, Shumay E, Ovod V, Erfle V. 2001
HIV-1 Nef co-localizes with the astrocyte-specific cytoskeleton protein GFAP in persistently nef-expressing human astrocytes. J Neurovirol. 7(1):52-5.

Collings, A., Pitkanen, J., Strengell, M., Tahtinen, M., Lagerstedt, A., Hakkarainen, K., Ovod, V., Sutter, G., Ustav, M., Ustav, E., Mannik, A., Ranki, A., Peterson, P., Krohn, K. 1999.
Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine. 18(5- 6):460-7.

Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren. 2001.
Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61.

Lehmann M.H., Walter S, Ylisastigui L, Striebel F, Ovod V, Geyer M, Gluckman JC, Erfle V. 2006.
Extracellular HIV-1 Nef increases migration of monocytes. Exp Cell Res. 312(18):3659-68

This product is for research use only


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