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Mouse mAb to Hev b6.02 (Hevein) (clone N3)

 

Figure 1. Western blot analysis demonstrating Hev b 6.02-N3 antibody binding only to a FL antigen with an apparent MW consistent with that of prohevein (20 kDa). Hev b 6.02 is the N-terminal region of prohevein (Hev b 6.01).

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€ 2098

Catalogue #

A3-710-100

Name

Mouse mAb to Hev b6.02 (Hevein) (clone N3)

Target

Hev b6.02

Target Description

Hev b6.02 - MBP fusion protein expressed and purified from E. coli.

Alternative Names

Hevein (Heveabrasiliensis), processed from pro-hevein precursor

Uniprot ID

P02877

Clonality

Mouse monoclonal

Clone

N3

Class

mIgG1

Reactivity

Recombinant and native Hev b6.02

Application

ELISA, WB

ELISA:

1:32 000 Reacts as detection antibody in capture ELISA with Hev b6.02 binding monoclonal antibody A3-709-100

Purification

Protein G purification

Buffer

PBS, 0.1% sodium azide

Related Products

A3-709-100 - mouse monoclonal antibody to Hev b6.02, used as a binding antibody in pair with detection antibody A3-710-100 in capture ELISA. Monoclonal antibodies to Hev b1, Hev b3 and Hev b5 are available.

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result

Background

Liquid latex from the rubber tree, Hevea brasiliensis, is the source of natural rubber latex (NRL) and contains over 200 proteins; 14 of them have been identified as allergens. Only some allergens retain their allergenic properties through the manufacturing processes. The NRL allergens that have been shown to be clinically relevant to genuine NRL allergy, and present in the final NRL products with maintained allergenicity are Hev b1, Hev b3, Hev b5 and Hev b6.02

References

ASTM D7427 - 08e1 Standard Test Method for Immunological Measurement of Four Principal Allergenic Proteins (Hev b 1, 3, 5 and 6.02) in Natural Rubber and Its Products Derived from Latex.

Koh D, Ng V, Leow YH, Goh CL. 2005.
A study of natural rubber latex allergens in gloves used by healthcare workers in Singapore. Br J Dermatol. 153(5):954-9.

Palosuo T, Alenius H, Turjanmaa K. 2002.
Quantitation of latex allergens. Methods. 27(1):52-8.

Peixinho C, Tavares-Ratado P, Tomás MR, Taborda-Barata L, Tomaz CT. 2008.
Latex allergy: new insights to explain different sensitization profiles in different risk groups. Br J Dermatol. 159(1):132-6.

Kang PB, Vogt K, Gruninger SE, Marshall M, Siew C, Meyer DM. 2007.
The immuno cross-reactivity of gutta percha points. Dent Mater. 23(3):380-4

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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