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Mouse mAb to Hev b3 (Small rubber particle protein, SRPP) (clone 5)

 

Figure 1. Western Blot testing of natural latex extract using anti-HevB1 antibody (A3-704-100, clone 5) Line 1. 10 µl of 20x diluted natural latex extract were loaded per line. Line 2. Protein size marker.

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Quantity:

€ 2098

Catalogue #

A3-704-100

Name

Mouse mAb to Hev b3 (Small rubber particle protein, SRPP) (clone 5)

Target

Hev b3

Target Description

Hev b3 - MBP fusion protein expressed and purified from E. coli.

Alternative Names

Small rubber particle protein - SRPP (Hevea brasiliensis)

Uniprot ID

O82803

Clonality

Mouse monoclonal

Clone

5

Class

mIgG1

Reactivity

Recombinant and native Hev b3

Application

ELISA, WB

Protocol

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

0.4 – 0.8 µg/ml. Reacts as binding antibody in capture ELISA with Hev b3 detection monoclonal antibody A3-705-100.

WB:

0.8 µg/ml

Purification

Protein G purification

Buffer

PBS, 0.1% sodium azide

Related Products

A3-705-100 - mouse monoclonal antibody to Hev b3, used as a detection antibody in pair with binding antibody A3-704-100 in capture ELISA. Monoclonal antibodies to Hev b1, Hev b5 and Hev b6.02 are available.

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result

Background

Liquid latex from the rubber tree, Hevea brasiliensis, is the source of natural rubber latex (NRL) and contains over 200 proteins; 14 of them have been identified as allergens. Only some allergens retain their allergenic properties through the manufacturing processes. The NRL allergens that have been shown to be clinically relevant to genuine NRL allergy, and present in the final NRL products with maintained allergenicity are Hev b1, Hev b3, Hev b5 and Hev b6.02.

References

ASTM D7427 - 08e1 Standard Test Method for Immunological Measurement of Four Principal Allergenic Proteins (Hev b 1, 3, 5 and 6.02) in Natural Rubber and Its Products Derived from Latex.

Koh D, Ng V, Leow YH, Goh CL. 2005.
A study of natural rubber latex allergens in gloves used by healthcare workers in Singapore. Br J Dermatol. 153(5):954-9.

Palosuo T, Alenius H, Turjanmaa K. 2002.
Quantitation of latex allergens. Methods. 27(1):52-8.

Peixinho C, Tavares-Ratado P, Tomás MR, Taborda-Barata L, Tomaz CT. 2008.
Latex allergy: new insights to explain different sensitization profiles in different risk groups. Br J Dermatol. 159(1):132-6.

Kang PB, Vogt K, Gruninger SE, Marshall M, Siew C, Meyer DM. 2007.
The immuno cross-reactivity of gutta percha points. Dent Mater. 23(3):380-4

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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