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Figure 1. Western Blot testing of natural latex extract using anti-Hev b1 antibody (A3-701-100, clone 5) Line 1. 10 µl of 20x diluted natural latex extract were loaded per line. Line 2. Protein size marker.
Mouse mAb to Hev b1 (Rubber elongation factor, REF) (clone 1-5)
Hev b1 - MBP fusion protein expressed and purified from E. coli.
Rubber elongation factor protein – REF (Hevea brasiliensis)
Recombinant and native Hev b1
0.05 – 0.1 µg/ml. Reacts as binding antibody in capture ELISA with Hev b1 detection monoclonal antibody A3-700-100
0.25 – 0.5 µg/ml
Protein G purification
PBS, 0.1% sodium azide
A3-701-100 - mouse monoclonal antibody to Hev b1, used as a binding antibody in pair with detection antibody A3-700-100 in capture ELISA. Monoclonal antibodies to Hev b3, Hev b5 and Hev b6.02 are available.
This product is shipped in non-frozen liquid form in ambient conditions
Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result
Liquid latex from the rubber tree, Hevea brasiliensis, is the source of natural rubber latex (NRL) and contains over 200 proteins; 14 of them have been identified as allergens. Only some allergens retain their allergenic properties through the manufacturing processes. The NRL allergens that have been shown to be clinically relevant to genuine NRL allergy, and present in the final NRL products with maintained allergenicity are Hev b1, Hev b3, Hev b5 and Hev b6.02.
ASTM D7427 - 08e1 Standard Test Method for Immunological Measurement of Four Principal Allergenic Proteins (Hev b 1, 3, 5 and 6.02) in Natural Rubber and Its Products Derived from Latex.
Koh D, Ng V, Leow YH, Goh CL. 2005.
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Peixinho C, Tavares-Ratado P, Tomás MR, Taborda-Barata L, Tomaz CT. 2008.
Latex allergy: new insights to explain different sensitization profiles in different risk groups. Br J Dermatol. 159(1):132-6.
Kang PB, Vogt K, Gruninger SE, Marshall M, Siew C, Meyer DM. 2007.
The immuno cross-reactivity of gutta percha points. Dent Mater. 23(3):380-4
This product is for research use only
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