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Mouse mAb to HCV NS5B (clone 8G6)

         

Figure 1. IP was carried out with NS5B specific mAb 8G6 using the lysates of Huh7 cells harboring selectable subgenomic HCV RNA replicon (upper panel) or plain Huh7 cells (lower panel). NS5B polyclonal antibodies (a-NS5B) and a-Bcl2 mAb, directed against cellular protein, were used as positive and negative controls respectively. Asterisk indicates immunoglobulin heavy chain; protein blots were probed with NS5B specific mAb 10D6

   

Figure 2. WB analysis of Huh-7 cell line expressing NS5B protein. Detected by 8G6 antibody. Line 1. Huh-7 transfected with HCV replicon, Line 2. Huh-7 non-transfected cells.

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Quantity:

€ 2098

Catalogue #

A2-428-100

Name

Mouse mAb to HCV NS5B (clone 8G6)

Target

HCV 1b NS5B

Target Description

Recombinant Hepatitis C Virus subtype 1b non-structural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)

Uniprot ID

Q9WMX2

Clonality

Mouse monoclonal

Clone

8G6

Class

mIgG1

Reactivity

Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 77-86 (TVKAKLLSVE)

Application

ELISA, WB, IP

Protocol

Optimal conditions for IP should be determined for each particular application.

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

WB:

in reduced conditions 1 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Related Products

HCV subtype 1b monoclonal antibodies. For more information visit www.icosagen.com/products/?antibodies

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Background

Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication.

References

Nikonov A, Juronen E, Ustav M (2008)
Functional characterization of fingers subdomain-specific monoclonal antibodies inhibiting the hepatitis C virus RNA-dependent RNA polymerase. J. Biol Chem. 283(35):24089-102.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com
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