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Figure 1. Western Blot testing of anti-BPV E2 monoclonal antibody (5E11).
A:Testing of purified recombinant BPV E2. Different amount of purified protein was loaded into the gel. Lane 1. 30 ng; Lane 2. 15 ng; Lane 3. 10 ng; Lane 4. 5 ng; Lane 5. 1 ng.
B: Testing of BPV E2 expressed in CHO cell line. Lane 1. Cell lysate of CHO cells. Lane 2. Cell lysate of CHO cells stably expressing BPV E2
Mouse mAb to BPV type 1 E2-protein (clone 5E11)
BPV type 1 E2
Recombinant full length BPV type 1 E2 protein purified from E. coli
Bovine, BPV type 1 E2 protein amino acids 199-208 (SSTSSDFRDR)
ELISA, WB, IF, IP
0.2 - 1.2 µg/ml
Protein G purification
PBS with 50% glycerol
9 different monoclonal antibodies to BPV type 1 E2 protein. For more information and ordering see full list: www.icosagen.com/products/?antibodies
This product is shipped in non-frozen liquid form in ambient conditions
Store at -20 or -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result
E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV
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This product is for research use only
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