Mouse mAb to BPV E2 ( clone 3H5)

Catalogue #

A1-153-100

Name

Mouse mAb to BPV E2 ( clone 3H5)

Target

BPV type 1 E2

Target Description

Recombinant full length BPV type 1 E2 protein purified from E. coli

Uniprot ID

P03122

Clonality

Mouse monoclonal

Clone

3H5

Class

IgG

Reactivity

Bovine, BPV type 1 E2 protein, mapped to amino acids310-410

Application

ELISA, IF

Protocol

ELISA:

0,1-0,2 µg/ml

WB:

-

IF:

0,5-20 µg/ml

Purification

Protein A purification

Buffer

PBS with 50% glycerol

Related Products

9 different monoclonal antibodies to BPV type 1 E2 protein. For more information and ordering see full list: www.icosagen.com/products/?antibodies

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Background

E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV

References

Kurg R, Parik J, Juronen E, Sedman T, Abroi A, Liiv I, Langel Ü, and Ustav M. 1999.
Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication, J Virol. 73: 4670-77.

Kurg R, Tekkel H, Abroi A, and Ustav M. 2006.
Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers, J Virol. 80(22): 11218-25