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Figure 1. Western Blot testing of anti-BPV E2 monoclonal antibody (1E4). Different amount of purified recombinant E2 protein was loaded. Lane 1. 15 ng; Lane 2. 10 ng; Lane 3. 5 ng; Lane 4. 1 ng
Figure 2. Immunofluorescence testing of anti-BPV E2 monoclonal antibody (1E4). Assay was carried out with U2OS cells expressing BPV E2 protein. Antibody concentration of 0.33 µg/ml was used. Nuclei were stained by DAPI. Fluorescence was measured at (A) 460 nm and 488 nm wavelengths or (B) 488 nm only.
Mouse monoclonal antibody to BPV type 1 E2 (clone 1E4)
BPV type 1 E2
Recombinant full length BPV type 1 E2 protein purified from E. coli
Bovine, BPV type 1 E2 protein, mapped to amino acids 250-280
ELISA, WB, IF
0.33 - 1 µg/ml
0 - 20 µg/ml
Protein A purification
PBS with 50% glycerol
9 different monoclonal antibodies to BPV type 1 E2 protein. For more information and ordering see full list: www.icosagen.com/products/?antibodies
This product is shipped in non-frozen liquid form in ambient conditions
Store at -20 °C to -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.
E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV
Kurg R, Parik J, Juronen E, Sedman T, Abroi A, Liiv I, Langel Ü, and Ustav M. 1999.
Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication, J Virol. 73: 4670-77.
Kurg R, Tekkel H, Abroi A, and Ustav M. 2006.
Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers, J Virol. 80(22): 11218-25
This product is for research use only
Please refer any technical questions email@example.com