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Mouse mAb to bovine DNase I (clone 6D5)

Mouse mAb to bovine DNase I (clone 6D5)
Figure 1. Western Blot testing of anti-bovine DNaseI monoclonal antibody (6D5). Lane 1. Recombinant bovine DNaseI expressed into the supernatant of CHO cell culture medium. 10 µl of supernatant was loaded. Lane 2. PageRuler Prestained Protein Ladder (#SM0671 Fermentas)

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Quantity:

€ 2307

Catalogue #

405-100

Name

Mouse mAb to bovine DNase I (clone 6D5)

Target

Bovine DNase I

Target Description

Recombinant full length bovine DNaseI produced using CHO-based Icosagen Cell Factory Ltd. proprietary suspension cell line. Antigen is purified from cell culture supernatant.

Alternative Names

DNL1

Uniprot ID

P00639

Clonality

Mouse monoclonal

Clone

6D5

Class

mIgG1

Reactivity

Bovine, cross reactivity to Human

Application

ELISA, WB

Protocol

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

ELISA:

0,01-0,02 µg/ml

WB:

0,1-0,2 µg/ml

IF:

0,33-20 µg/ml

Purification

Protein G purification

Buffer

PBS, pH 7.4

Shipping

Shipping in non-frozen liquid form

Storage

Store at -20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Background

Bovine deoxyribonuclease I (DNase I) degrades single- and double-stranded DNA. The enzyme works by cleaving DNA into 5' phosphodinucleotide and small oligonucleotide fragments.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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