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Mouse mAb to bovine DNase I (clone 4B10)

Mouse mAb to bovine DNase I (clone 4B10)
Figure 1. Western Blot testing of anti-bovine DNaseI monoclonal antibody (4B10). Lane 1. Recombinant bovine DNaseI expressed into the supernatant of CHO cell culture medium. 10 µl of cell culture supernatant was loaded into the gel. Lane 2. PageRuler Prestained Protein Ladder (#SM0671 Fermentas)

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Quantity:

€ 2307

Catalogue #

404-100

Name

Mouse mAb to bovine DNase I (clone 4B10)

Target

Bovine DNase I

Target Description

Recombinant full length bovine DNaseI produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Antigen is purified from cell culture supernatant

Alternative Names

DNL1

Uniprot ID

P00639

Clonality

Mouse monoclonal

Clone

4B10

Class

mIgG1

Reactivity

Bovine, cross reactivity to Human

Application

ELISA, WB

Protocol

Antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

0,01-0,02 µg/ml

WB:

0,1-0,2 µg/ml

IF:

-

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20… -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result

Background

Bovine deoxyribonuclease I (DNase I) degrades single- and double-stranded DNA. The enzyme works by cleaving DNA into 5' phosphodinucleotide and small oligonucleotide fragments

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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