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Human IgG1-lambda antibody to SARS CoV-2 Spike S1 (clone 8E5)

 

Figure 1. Coomassie-stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Spike S1, clone 8E5. 4-12% gradient gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Spike S1, clone 8E5 (-DTT). Lane 2. Size Marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Spike S1, clone 8E5 (+DTT). Lane 4. Size Marker.

       

Figure 2. HPLC analytical SEC for final product.

       

Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.

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Catalogue #

R1-163-100

Name

Human IgG1-lambda antibody to SARS CoV-2 Spike S1 (clone 8E5)

Target

SARS-CoV-2 virus

Target Description

SARS-CoV-2 virus (PBMCs isolated from patients recovered from SARS-CoV-2 infection, antibodies discovered by using HybriFree Technology).

Clonality

Human monoclonal

Clone

8E5

Class

hIgG1

Reactivity

SARS-CoV-2 Spike S1

Dissociation constant (KD)

< 1.0 x 10-12 M (measured against SARS-CoV-2 Trimeric Spike protein)

9.70 x 10-9 M (measured against SARS-CoV-2 Spike S1 protein)

Application

ELISA

Protocol

Indirect ELISA 0.02 – 0.04 ng/ml

Kd:

< 1.0 x 10-12 M (measured against SARS-CoV-2 Trimeric Spike protein) 9.70 x 10-9 M (measured against SARS-CoV-2 Spike S1 protein)

ELISA:

indirect ELISA 0,02-0,04 ng/ml

Purification

Protein A affinity chromatography following desalting

Buffer

PBS pH 7.4

Unit Size

100 µg

Shipping

Shipped on dry ice

Storage

Store at -20 °C to -70 °C long term. Avoid multiple freeze-thaw cycles.

Limitations

This product is for research use only.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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