Search from website

Human IgG1-lambda antibody to SARS CoV-2 S1 RBD (clone 28H3)

 

Figure 1. Coomassie-stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Spike RBD, clone 28H3. 4-12% gradient gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 28H3 (-DTT). Lane 2. Size Marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 28H3 (+DTT).

       

Figure 2. HPLC analytical SEC for final product.

       

Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.

     

 

Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of Spike S1 protein.

Select volume:

Quantity:

Catalogue #

R1-156-100

Name

Human IgG1-lambda antibody to SARS CoV-2 S1 RBD (clone 28H3)

Target

SARS-CoV-2 virus

Target Description

SARS-CoV-2 virus (PBMCs isolated from patients recovered from SARS-CoV-2 infection, antibodies discovered by using HybriFree Technology).

Clonality

Human monoclonal

Clone

28H3

Class

hIgG1

Reactivity

SARS-CoV-2 Spike RBD

Dissociation constant (KD)

< 1.0 x 10-12 M (measured against SARS-CoV-2 Trimeric Spike protein)

3.22 x 10-9 M (measured against SARS-CoV-2 Spike S1 protein)

Application

ELISA

Protocol

Indirect ELISA 0.02 – 0.04 ng/ml

Kd:

< 1.0 x 10-12 M (measured against SARS-CoV-2 Trimeric Spike protein)3.22 x 10-9 M (measured against SARS-CoV-2 Spike S1 protein)

ELISA:

indirect ELISA 0,02-0,04 ng/ml

Purification

Protein A affinity chromatography following gel filtration

Concentration

1 mg/ml

Buffer

PBS pH 7.4

QC

SDS-PAGE, analytical SEC for final product and after 3 freeze-thaw cycles,

Unit Size

100 µg

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20 °C to -70 °C long term. Avoid multiple freeze-thaw cycles.

Limitations

This product is for research use only.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

Our locations

United States Office

77 Geary St. 5th Floor
San Francisco, CA 94108
+1 415 548 6625

European Office

Eerika tee 1
Õssu, Kambja vald
Tartu maakond 61713
Estonia
+372 737 7070
error:
Shopping Cart