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Figure 1. Coomassie-stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Spike RBD, clone 28H3. 4-12% gradient gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 28H3 (-DTT). Lane 2. Size Marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 28H3 (+DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of Spike S1 protein.
Catalogue #
R1-156-100
Name
Human IgG1-lambda antibody to SARS CoV-2 S1 RBD (clone 28H3)
Target
SARS-CoV-2 virus
Target Description
SARS-CoV-2 virus (PBMCs isolated from patients recovered from SARS-CoV-2 infection, antibodies discovered by using HybriFree Technology).
Clonality
Human monoclonal
Clone
28H3
Class
hIgG1
Reactivity
SARS-CoV-2 Spike RBD
Dissociation constant (KD)
< 1.0 x 10-12 M (measured against SARS-CoV-2 Trimeric Spike protein)
3.22 x 10-9 M (measured against SARS-CoV-2 Spike S1 protein)
Application
ELISA
ELISA:
indirect ELISA 0,02-0,04 ng/ml
Purification
Protein A affinity chromatography following gel filtration
Concentration
1 mg/ml
Buffer
PBS pH 7.4
QC
SDS-PAGE, analytical SEC for final product and after 3 freeze-thaw cycles,
Shipping
Shipped at ambient temperature.
Storage
Store at +4 °C. Avoid multiple freeze-thaw cycles.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
© 2025 Icosagen
