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Figure 1. Simply Blue Safe stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Spike RBD, clone 72A5. 4-12% gradient gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 72A5 (-DTT). Lane 2. Size marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 72A5 (+DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of Spike S1 protein in different concentrations (200, 100, 50, 25 and 12.5 nM).
Figure 5. Octet RED96e analysis, antibody was loaded on sensor for capture of Trimeric Spike protein in different concentrations (50, 25, 12.5, 6.25 and 3.13 nM).
Catalogue #
R1-172-100
Name
Rabbit IgG-kappa antibody to SARS-CoV-2 S1 RBD (clone 72A5)
Target
RBD protein SARS-CoV-2
Target Description
Recombinant SARS-CoV-2 RBD protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line
Clonality
Rabbit monoclonal
Clone
72A5
Class
rIgG1
Reactivity
SARS-CoV-2 Spike RBD
Dissociation constant (KD)
<1.0 x 10-12 M (measured against SARS-CoV-2 Trimeric Spike protein)
5.16 x 10-9 M (measured against SARS-CoV-2 Spike S1 protein)
Application
ELISA
ELISA:
0,005-0,01 ng/ml
Purification
Protein A affinity chromatography following gel filtration
Buffer
PBS pH 7.4
Shipping
Shipped at ambient temperature.
Storage
Store at +4 °C. Avoid multiple freeze-thaw cycles.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
© 2024 Icosagen
