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Human IgG1-lambda antibody to SARS CoV-2 S1 RBD (clone 60E10)

 

Figure 1. Coomassie-stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Spike RBD, clone 60E10. 10% gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 60E10 (+DTT). Lane 2. Size marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 60E10 (-DTT).

       

Figure 2. HPLC analytical SEC for final product.

       

Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.

       

Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of Spike S1 protein in different concentrations: 200 nM, 100 nM, 50 nM, 25 nM and 12.5 nM.

 

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Catalogue #

R1-174-100

Name

Human IgG1-lambda antibody to SARS CoV-2 S1 RBD (clone 60E10)

Target

SARS-CoV-2 virus

Target Description

SARS-CoV-2 virus (PBMCs isolated from patients recovered from SARS-CoV-2 infection, antibodies discovered by using HybriFree Technology).

Clonality

Human monoclonal

Clone

60E10

Class

hIgG1

Reactivity

SARS-CoV-2 Spike RBD
Neutralizing properties: blocks S1 and ACE2 binding in Octet system; IC50 125 ng/ml measured in live virus neutralization assay

Dissociation constant (KD)

<1.0 x 10-12 M (measured against SARS-CoV-2 Trimeric Spike protein)

2.22 x 10-9 M (measured against SARS-CoV-2 Spike S1 protein)

Application

ELISA

ELISA:

0,02-0,04 ng/ml

Purification

Protein A affinity chromatography following gel filtration

Buffer

PBS pH 7.4

Shipping

Shipped at ambient temperature.

Storage

Store at -20 °C to -70 °C long term. Avoid multiple freeze-thaw cycles.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com
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