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Human IgG1-lambda antibody to SARS CoV-2 S1 RBD (clone 36A7)

Human IgG1-lambda antibody to SARS CoV-2 S1 RBD (clone 36A7)  

Figure 1. Coomassie-stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Spike RBD, clone 36A7. 4-12% gradient gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 36A7 (-DTT). Lane 2-3. Size marker. Lane 4. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 36A7 (+DTT).

      Human IgG1-lambda antibody to SARS CoV-2 S1 RBD (clone 36A7)  

Figure 2. HPLC analytical SEC for final product.

      Human IgG1-lambda antibody to SARS CoV-2 S1 RBD (clone 36A7)  

Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.

      Human IgG1-lambda antibody to SARS CoV-2 S1 RBD (clone 36A7)  

Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of Spike S1 protein (blue line) and trimeric Spike protein (red line).

         

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Catalogue #

R1-164-100

Name

Human IgG1-lambda antibody to SARS CoV-2 S1 RBD (clone 36A7)

Target

RBD protein SARS-CoV-2

Target Description

Recombinant SARS-CoV-2 RBD2 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line

Clonality

Human monoclonal

Clone

36A7

Class

hIgG1

Reactivity

SARS-CoV-2 Spike RBD
Neutralizing properties: blocks S1 and ACE2 binding in Octet system; IC50 1250 ng/ml measured in live virus neutralization assay

Dissociation constant (KD)

6.27 x 10-12 M (measured against SARS-CoV-2 Trimeric Spike protein)

5.10 x 10-8 M (measured against SARS-CoV-2 Spike S1 protein)

Application

ELISA

ELISA:

0,04-0,08 ng/ml

Purification

Protein A affinity chromatography following desalting

Buffer

PBS pH 7.4

Shipping

Shipped at ambient temperature.

Storage

Store at +4 °C. Avoid multiple freeze-thaw cycles.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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