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Human IgG1 antibody to SARS CoV-2 Spike protein (clone 58F8)

 

Figure 1. Simply Blue Safe stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Spike RBD, clone 58F8. 4-12% gradient gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 58F8 (-DTT). Lane 2. Size marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 58F8 (+DTT).

       

Figure 2. HPLC analytical SEC for final product.

       

Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.

       

Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of Spike S1 protein in different concentrations (200, 100, 50, 25 and 12.5 nM).

       

Figure 5. Octet RED96e analysis, antibody was loaded on sensor for capture of Trimeric Spike protein in different concentrations (50, 25, 12.5, 6.25 and 3.13 nM).

               

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Catalogue #

R1-170-100

Name

Human IgG1 antibody to SARS CoV-2 Spike protein (clone 58F8)

Target

SARS-CoV-2 virus

Target Description

SARS-CoV-2 virus (PBMCs isolated from patients recovered from SARS-CoV-2 infection, antibodies discovered by using HybriFree Technology).

Clonality

Human monoclonal

Clone

58F8

Class

hIgG1

Reactivity

SARS-CoV-2 Spike RBD

Dissociation constant (KD)

< 1.0 x 10-12 M (measured against SARS-CoV-2 Trimeric Spike protein)

1.4 x 10-8 M (measured against SARS-CoV-2 Spike S1 protein)

Application

ELISA

ELISA:

0,01-0,02 ng/ml

Purification

Protein A affinity chromatography following gel filtration

Buffer

PBS pH 7.4

Shipping

Shipped at ambient temperature.

Storage

Store at -20 °C to -70 °C long term. Avoid multiple freeze-thaw cycles.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com
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