Human IgG1 antibody to SARS CoV-2 Spike protein (clone 58F8)

Figure 1. Simply Blue Safe stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Spike RBD, clone 58F8. 4-12% gradient gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 58F8 (-DTT). Lane 2. Size marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 58F8 (+DTT).

Figure 2. HPLC analytical SEC for final product.

Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.

Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of Spike S1 protein in different concentrations (200, 100, 50, 25 and 12.5 nM).

Figure 5. Octet RED96e analysis, antibody was loaded on sensor for capture of Trimeric Spike protein in different concentrations (50, 25, 12.5, 6.25 and 3.13 nM).